Understanding Analytical Testing for Peptides

An overview of common characterization techniques — HPLC, mass spectrometry and amino-acid analysis.

Peptide characterization usually combines several orthogonal techniques.

Reversed-phase HPLC

Separates components by hydrophobicity and is commonly used to estimate chromatographic purity.

Confirms molecular mass and therefore supports identity.

Helps establish composition and net peptide content.

Demonstration article — pending scientific review before publication.